Objective To transfect the dog bone mesenchymal stem cells (BMSCs) with lentivirus-mediated vascular endothelial growth factor 165 (VEGF165) and bone morphogenetic protein 2 (BMP-2) and observe the expressions of VEGF165 and BMP-2 in BMSCs.Methods Lentivirus vectors carrying VEGF165 and BMP-2 genes were constructed and used to transfect dog third-generation BMSCs,including untransfected group,VEGF165 group,BMP-2 group and double-gene (VEGF165 + BMP-2) group.Expressions of VEGF165 and BMP-2 mRNA were detected by RT-PCR 7 d after the transfection.Expressions of VEGF165 and BMP-2 protein were detected by Western blotting 5 d and 28 d after the transfection.Cell growth was detected by MTT assay.Results RT-PCR showed high expression of VEGF165 mRNA in VEGF165 and double-gene groups,and BMP-2 mRNA in BMP-2 and double-gene groups,but there was no significant difference in group comparison (P ＞ 0.05).Western blotting showed specific performance of VEGF165 protein in VEGF165 and double-gene groups,and BMP-2 protein in BMP-2 and double-gene groups.In MTT assay cells were found to have 2-3 d relatively stationary phase in the early period,proliferated rapidly at 3 d,reaching the logarithmic growth,and entered the plateau phase with slow propagation.There was no significant difference in absorbance value before and after the transfection (P ＞ 0.05).Conclusion BMSCs with highly expressed VEGF165 and BMP-2 can be successfully obtained by lentiviral transduction.