Abstract：Objective To investigate correlation between demyelination and caspase-12 expression alteration after compressed spinal cord injury (CSCI) so as to discuss mechanism of demyelinating lesion after CSCI. Methods Seventy-five adult SD rats were randomly divided into five groups, ie, normal group, control group, compression 1 d, 3 d and 7 d groups, with 15 rats per group. Models of spinal cord compression were established with a self-made device. Ultrastructure of the demyelinated nerve fibers was observed by electronic microscope and oligodendrocyte apoptosis was detected by TUNEL staining and double labeling immunofluorescence. Immunoblotting was used to defect caspase-12 that was related to cell apoptosis. Results Demyelination of nerve fiber occurred after CSCI and was aggravated with time. Apoptosis of oligodendrocytes was found after CSCI, and showed significant difference between compression 7 d group and normal group (P<0.05). Caspase-12 was also up-regulated with extension of compression time. Conclusion Caspase-12 mediating oligodendrocyte apoptosis is one of the mechanisms of nerve fiber demyelination after CSCI.
HUANG Si-qin,QI Wei,SUN Shan-quan et al. Expression alteration of caspase12 and demyelination after compressed spinal cord injury[J]. CHINESE JOURNAL OF TRAUMA, 2013, 29(2): 160-164.
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