Abstract：Objective To evaluate the degenerative regularity of motoneurons in anterior horn of spinal cord after unilateral sacral plexus avulsion in rats. Methods Sixty adult SD rats weighing 200-300 g were chosen to establish models of unilateral sacral plexus avulsion. Spinal cord segments of L4-6 were removed and sectioned at postinjury 2, 4, 6, 8, 10, 12 weeks respectively to detect survival rate of motoneurons in anterior horn of spinal cord using HE staining and to detect their apoptotic index using TUNNEL staining. Results Survival rates of motoneurons at weeks 2, 4, 6, 8, 10, 12 after avulsion were (92.1±4.7)％, (83.6±3.7)％, (43.6±4.2)％, (32.1±3.5)％, (18.4±3.7)％, and (12.1±3.3)％, respectively. Consequently, survival rate of motoneurons in anterior horn of spinal cord was decreased gradually as the expansion of injury time, but their apoptotic index presented a gradual increase with time, especially at 6 weeks after injury. Conclusion Six weeks after sacral plexus avulsion in rats may be taken as the time node of changes of motoneurons in anterior horn of spinal cord and nerve repair should be completed in advance of the time node.
JIANG Xi,CHEN Ai-min,YANG Di et al. Degeneration law of anterior horn of spinal cord in rats with sacral plexus avulsion[J]. CHINESE JOURNAL OF TRAUMA, 2013, 29(2): 156-159.
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