大鼠急性肺损伤病理变化的形态计量研究

doi:10.3760/cma.j.issn.1001-8050.2013.4.018

摘要
图/表
参考文献(15)
相关文章 (3)

全文: PDF (0 KB) HTML (0 KB)
输出: BibTeX | EndNote (RIS) 背景资料

摘要目的用体视学方法定量研究大鼠经胸部撞击复合内毒素所致急性肺损伤（acute lung injury， ALI）模型的组织病理学特点及变化规律。方法 12只SD大鼠按随机数字表法分为正常对照组、ALI致伤1，2，7 d组，每组3只。各组取肺组织行HE染色，分别测试肺实质百分比、肺泡间隔厚度、肺泡截面积及肺泡腔内炎性细胞计数。结果 ALI致伤1，2 d组肺泡腔内炎性细胞计数分别为（13.08±6.60）个、（26.81±16.86）个，正常对照组为（0.89±0.78）个，致伤组均较对照组明显增加(P<0.01)。ALI致伤1，2，7 d组肺实质百分比分别为（23.77±3.91）%、（27.32±5.95）%、（44.57±6.46）%，正常对照组为（19.44±2.48）%，致伤组均较对照组明显增加(P<0.01)。ALI致伤1，2，7 d组肺泡间隔分别为（3.50±2.18）μm、（7.67±7.14）μm、（17.23±13.08）μm，正常对照组为（2.87±1.75）μm，致伤组均较对照组逐渐增厚(P< 0.05)。ALI致伤1,2,7 d组肺泡截面积分别为（1 653.90±950.77）μm2、（1 328.15±802.30）μm2、（1 055.20±1 002.75）μm2，正常对照组为（1 641.30±906.60）μm2，致伤组均较对照组逐渐减少(P< 0.05)。结论大鼠经胸部撞击后复合静脉注射内毒素可建立病理形态上符合ALI的动物模型。通过体视学方法定量检测可反映ALI的组织病理学特点及变化规律。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	王海燕
	赵玉金
	杜娟
	李海胜
	杨策
	詹俊
	岳彩黎
	蒋建新

关键词 ：急性肺损伤, 模型, 动物, 形态计量

Abstract：Objective To quantitatively study the histopathological features and its variation rules in rat models of acute lung injury (ALI) induced by chest impact accompanied with endotoxin infection by stereological method. Methods Twelve SD rats were randomly divided into control group and groups at 1, 2, and 7 days after ALI, with 3 rats per group. HE staining of lung tissue specimens was performed for each group so as to detect percentage of lung parenchyma, thickness of alveolar septum, alveolar section area and count of inflammatory cells in alveolar space. Results Inflammatory cells in alveolar space in groups at 1 and 2 days after ALI and control group counted 13.08±6.60, 26.81± 16.86 and 0.89±0.78 respectively, with a far higher number in the injured groups (P< 0.01). Percentage of lung parenchyma in groups at 1, 2, and 7 days after ALI and control group was (23.77±3.91)%, (27.32±5.95)%, (44.57±6.46)% and (19.44±2.48)% respectively, with a far higher proportion in the injured groups (P< 0.01). Alveolar septum in the groups at 1 and 2 days after ALI and control group was (3.50±2.18) μm, (7.67±7.14) μm, (17.23±13.08) μm, and (2.87± 1.75) μm respectively, with a gradual thickening in the injured groups (P< 0.05). Alveolar section area in groups at 1, 2, and 7 days after ALI and control group was (1 653.90±950.77) μm2, (1 328.15±802.30) μm2, (1 055.20±1 002.75) μm2, and (1 641.30±906.60) μm2 respectively, with a gradual decline in the injured groups (P< 0.05). Conclusions Chest impact with intravenous administration of endotoxin on rats sets up models that accord with ALI in pathomorphology successfully. Quantitative study by stereological method reflects features and variation rules of histopathology of ALI.

Key words： Acute lung injury Models, animal Morphometry

基金资助:国家重点基础研究发展计划资助项目（2012CB518104）；创伤、烧伤与复合伤国家重点实验室自主研究课题资助项目（SKLZZ201104）

引用本文:

王海燕,赵玉金,杜娟等. 大鼠急性肺损伤病理变化的形态计量研究[J]. 中华创伤杂志, 2013, 29(4): 364-367.

WANG Hai-yan,ZHAO Yu-jin,DU Juan et al. Morphometrical study of pathological changes in rats with acute lung injury[J]. CHINESE JOURNAL OF TRAUMA, 2013, 29(4): 364-367.

链接本文:

http://www.zhcszz.com/CN/10.3760/cma.j.issn.1001-8050.2013.4.018 或 http://www.zhcszz.com/CN/Y2013/V29/I4/364

	［1］廖克龙, 朱佩芳, 王正国, 等. 胸部撞击伤、内毒素血症及其复合损伤对大鼠肺血管通透性的影响. 第三军医大学学报, 2001, 23（2）:199-200.
	［2］Gupta N, Su X, Popov B, et al. Intrapulmonary delivery of bone marrow-derived mesenchymal stem cells improves survival and attenuates endotoxin-induced acute lung injury in mice. J Immunol, 2007, 179(3):1855-1863.
	［3］初向阳, 侯晓彬, 张连斌, 等. 兔胸部爆炸伤后血液高凝状态的实验研究. 创伤外科杂志, 2010, 12（2）:165-168.
	［4］Hsia CC, Hyde DM, Ochs M, et al. An official research policy statement of the American Thoracic Society/European Respiratory Society: standards for quantitative assessment of lung structure. Am J Respir Crit Care Med, 2010, 181（4）:394-418.
	［5］Ochs M. Stereological analysis of acute lung injury. Eur Respir Rev, 2006, 15（101）:115-121.
	［6］McCarter SD, Mei SH, Lai PF, et al. Cell-based angiopoietin-1 gene therapy for acute lung injury. Am J Respir Crit Care Med, 2007, 175（10）:1014-1026.
	［7］Ghezzo RH. An Introduction to lung Morphometry//Hamid Q, Shannon T, Martin J. physiological basis of respiratory disease. 1st ed. Shelton: PMPH-USA, 2005:769-777.
	［8］荣健, 叶升, 梁孟亚, 等. 体视学定量法评估氧控制性再灌注对犬体外循环肺缺血再灌注早期损伤的保护作用. 中国生物医学工程学报, 2011, 30（5）:690-694.
	［9］Yelveton JT. Pathology scoring system for blast injuries. J Trauma, 1996, 40 (3 Suppl) S111-S115.
	［10］Rocco PR, Zin WA. Pulmonary and extrapulmonary acute respiratory distress syndrome: are they different? Curr Opin Crit Care, 2005, 11（1）:10-17.
	［11］Rubenfeld GD, Caldwell E, Peabody E. Incidence and outcomes of acute lung injury. N Engl J Med, 2005, 353（16）:1685-1693.
	［12］Matthay MA, Zimmerman GA, Esmon C, et al. Future research directions in acute lung injury. Am J Respir Crit Care Med, 2003, 167（7）:1027-1035.
	［13］Finkelstein JN, O’Reilly MA, Holm BA, et al. Cell and animal models of lung injury// Notter RH, Finkelstein JN, Holm BA, et al. Lung injury: mechanisms, pathophysiology, and therapy (lung biology in health and disease). 1st ed. Boca Raton:CRC Press of Taylor and Francis, 2005:353-404.
	［14］Takao Y, Mikawa K, Nishina K, et al. Attenuation of acute lung injury with propofol in endotoxemia. Anesth Analg, 2005, 100（3）:810-816.
	［15］Luce JM. Acute lung injury and the acute respiratory distress syndrome. Crit Care Med, 1998, 26 (2): 369-376.