Abstract：ObjectiveTo establish a model of multiple trauma combined with uncontrolled hemorrhagic shock so as to provide basis for basic study of multiple trauma.MethodsForty New Zealand male rabbits were equally and randomly divided into four groups, ie, sham group (Group A, with no bloodletting or resuscitation), uncontrolled group (Group B, with bloodletting alone), aggressive fluid resuscitation and limited fluid resuscitation groups (Groups C and D, with bloodletting, hemostasis and resuscitation). Fractures of the right hind limb in rabbits of each group were induced by free drop of a 3 kg hammer from a height of 45 cm. An estimated 30% of total blood was withdrawn from the right common carotid artery in 20 minutes in bloodletting groups (Groups B, C and D). Successively, the uncontrolled bleeding was caused via acupuncture bloodletting from ileocecal artery branch at 30 minutes in bloodletting groups. Progressive and limited fluid resuscitation using lactated Ringer’s solution （LR） were performed for Groups C and D in the next one hour respectively. Blood transfusion was performed in Groups C and D after ligation of bleeding artery branch at 90 minutes. In addition, LR of three folds more than blood loss was administered in Group D. Survivorship of rabbits in each group was observed at 150 minutes to seven hours. Arterial blood sample was taken at each time point for blood gas analysis and coagulation test. Fracture type, fluid requirements, intraabdominal blood loss, and rabbits’ survival rate at seven hours were recorded.ResultsClosed comminuted tibiofibular fractures were founded in all groups. Bloodletting groups showed an obvious reduction of MAP, heart rate and pH value and significantly increased levels of K+ , blood glucose and lactic acid at 30 minutes (P＜0.01), whereas MAP and heart rate were returned to baseline level at 150 minutes. Progressive fluid resuscitation significantly increased the intraabdominal blood loss and fluid requirements（P＜0.01）, decreased erythrocrit （P＜0.05）, prolonged prothrombin time and activated partial thromboplastin time （P＜0.05）. Survival rate of rabbits was improved significantly by fluid resuscitation at 7 hours （P＜0.01）.ConclusionThe model is of high stability and reproducibility and therefore is fit for study of multiple trauma combined with uncontrolled hemorrhagic shock.
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