Abstract：Objective To assess the clinical relevance of polymorphisms at position-251 in the promoter region of IL-8 gene and the incidence of sepsis in patients with severe trauma.Methods A total of 296 patients with severe trauma were included in the prospective cohort study.Incidence of sepsis was decided on the basis of the clinical manifestations and blood culture results.Muhiple organ dysfunction score (MODS) was performed using Marshall' s standard.IL-8/-251 gene polymorphisms were genotyped using restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP).IL-8 plasma level was determined using ELISA method.Results Genotype frequency at IL-8/-251 locus in trauma patients was in accord with Hardy-Weinberg equilibrium (P ＞ 0.05).Sepsis incidence in trauma patients with TT,TA,and AA genotypes at IL-8/-251 locus was 58.1％,49.6％,and 25.0％ respectively.Multiple regression analysis showed inverse correlation between sepsis incidence and quantity of A alleles [OR =0.637,95％ CI (0.421,0.963),P ＜ 0.05].Carriers of AA genotype presented lower MODS score than those of other two genotypes (P ＜ 0.05).IL-8 plasma level presented significant difference among carriers of the three genotypes (P ＜ 0.01) and A alleles were associated with the down-regulation of IL-8 (P ＜0.01).Conclusion IL-8/-251T/A polymorphisms are implicated in the development of posttraumatic sepsis and AA genotype is protective against posttraumatic sepsis.
. Relationship of IL-8/-251 gene polymorphisms with incidence of sepsis in patients with severe traumatic injury[J]. CHINESE JOURNAL OF TRAUMA, 2013, 29(12): 1220-1223.
［1］Harada A， Sekido N， Akahoshi T， et al. Essential involvement of interleukin-8 (IL-8) in acute inflammation. J Leukoc Biol， 1994， 56（5）:559-564.
［2］Fujishima S， Sasaki J， Shinozawa Y， et al. Serum MIP-1 alpha and IL-8 in septic patients. Intensive Care Med， 1996， 22（11）:1169-1175.
［3］Hotchkiss RS， Karl IE. The pathophysiology and treatment of sepsis. N Engl J Med， 2003， 348（2）:138-150.
［4］El-Maghraby SM， Moneer MM， Ismail MM， et al. The diagnostic value of C-reactive protein， interleukin-8， and monocyte chemotactic protein in risk stratification of febrile neutropenic children with hematologic malignancies. J Pediatr Hematol Oncol， 2007， 29（3）:131-136.
［5］Rodríguez-Wilhelmi P,Montes R,Matsukawa A,et al.Interleukin (IL)-8 and growth related oncogene-alpha in severe endotoxemia and the effects of a tumor necrosis factor-alpha/IL-1beta inhibitor on these chemokines.Exp Mol Pathol,2002,73(3):220-229.
［6］Fujishima S,Sasaki J,Shinozawa Y,et al. Serum MIP-1 alpha and IL-8 in septic patients. Intensive Care Med， 1996， 22(11):1169-1175.
［7］Mukaida N,Shiroo M， Matsushima K. Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8. J Immunol， 1989， 143(4):1366-1371.
［8］Hull J,Thomson A,Kwiatkowski D. Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. Thorax， 2000， 55(12):1023-1027.
［9］Kim JM， Stewart R， Kim SY， et al. A one year longitudinal study of cytokine genes and depression in breast cancer. J Affect Disord， 2013， 148(1):57-65. 〖ZK)〗
［10］Wang N， Zhou R， Wang C， et al. -251 T/A polymorphism of the interleukin-8 gene and cancer risk: a HuGE review and meta-analysis based on 42 case-control studies. Mol Biol Rep， 2012， 39(3):2831-2841.
［11］Liu S， Yin C， Chu N， et al. IL-8 -251T/A and IL-12B 1188A/C polymorphisms are associated with gout in a Chinese male population. Scand J Rheumatol， 2013， 42(2):150-158.
［12］Lu A， Wang L， Zhang X. Haplotype of IL-8 -251T and 781C is associated with the susceptibility to respiratory syncytial virus. J Trop Pediatr， 2010， 56(4):242-246.
