Abstract:Objective To observe the expressions of efflux pump gene cluster adeAB in acinetobacter baumannii isolated from the burn patients and the expression changes of its upstream regulatory genes adeR and adeS and determine the influence of those genes on drug resistance of acinetobacter baumannii.Methods Nine drug-resistant strains and nine sensitive strains of acinetobacter baumannii isolated from the burn patients treated between June 2012 and March 2013 were used.After strain identification using 16SrDNA sequencing,acinetobacter baumannii standard strain ATCC19606 was employed as the control.mRNA expressions of efllux pump genes adeA and adeB and their upstream regulatory genes adeR and adeS were detected by real-time quantitative PCR.Results (1) adeA and adeB genes presented higher expressions in drug-resistant strains (3.71 ±0.95,76.16 ± 8.75) than in sensitive strains (0.92 ± 0.94,0.72 ± 0.78) (F =38.71,663.65 respectively,both P < 0.05).(2) adeS and adeR genes showed higher expressions in drug resistant strains (18.02 ± 6.71,3.02 ± 2.69) than in sensitive strains (1.64±1.51,0.76±0.61) (F =51.04,5.57 respectively,bothP<0.05).Conclusions The over-expression of efflux pump gene cluster adeAB induced by the expression alteration of its upstream regulatory genes adeR and adeS is closely associated with the drug resistance of acinetobacter baumannii in the burn patient.Besides,the regulatory genes may depend on adeS to sense the nosocomial environment condition,activate or inactivate adeR and hence regulate efflux pump expression.
XIANG Jun,DOU Yi,ZHU Cai-lian et al. Relationship of drug resistance in acinetobacter baumannii isolated from the burn patients with the expression alteration of efflux pump adeAB[J]. CHINESE JOURNAL OF TRAUMA, 2013, 29(10): 915-918.
[2]Peleg AY, Adams J, Paterson DL. Tigecycline efflux as a mechanism for nonsusceptibility in acinetobacter baumannii. Antimicrob Agents Chemother,2007, 51(6):2065-2069.
[3]Keen EF 3rd,Robinson BJ,Hospenthal DR,et al. Prevalence of multidrug-resistant organisms recovered at a military burn center. Burns,2010,36(6):819-825.
[4]Hou PF,Chen XY,Yan GF, et al. Study of the correlation of imipenem resistance with efflux pumps AdeABC, AdeIJK, AdeDE and AbeM in clinical isolates of acinetobacter baumannii. Chemotherapy, 2012, 58(2):152-158.
[5]Wieczorek P, Sacha P, Hauschild T, et al. Multidrug resistant Acinetobacter baumannii-the role of adeABC (RND family) efflux pump in resistance to antibiotics. Folia Histochem Cytobiol,2008,46(3):257-267.
[6]Ruzin A, Immermann FW, Bradford PA. RT-PCR and statistical analyses of adeABC expression in clinical isolates of Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Microb Drug Resit,2010,16(2):87-89.