Abstract：Objective To investigate relationship between the variation degree of platelet mitochondria in rats with severe hemorrhagic shock and the degree of shock. Methods Thirty-six Wistar rats were divided into sham group, shock 30, 60, and 120 minutes groups, shock 120 minutes+normal saline (NS)+blood reinfusion group (NS group) and shock 120 minutes+polydatin (PD) + blood reinfusion group (PD group) according to random number table, with six rats per group. Content of ATP in platelets was detected by fluorescein-luciferase assay kit; structure of platelet mitochondria by electron microscope; state of mitochondrial permeability transition pore by Calcein-AM and CoCl2; change of mitochondrial membrane potential （△ψm） by JC-1 mitochondrial membrane potential kit; lipid hydroperoxide (LPO) in platelets by LPO assay kit; stability of platelet lysosomes by acridine orange (AO). Results ATP released from platelets was reduced significantly in shock 60 minutes group (P<0.01) and with the prolong of shock period, further reduction was observed, particularly in NS group [(50.75±9.15)% of normal value]. Mitochondrial swelling with poorly defined crista structure, declined △ψm, and low lysosome stability (pale cells were increased) were observed in shock 30 minutes group. Calcein fluorescence in mitochondria was faded in shock 60 minutes group (P<0.01). Whereas in PD group, all the above indices presented some recovery with ATP level returned to nearly (79.57±8.48)% of normal value in particular. Conclusions Platelet mitochondrial dysfunction takes place at 30-60 minutes following severe shock. Hence, it may be served as an non-invasive index for the diagnose and treatment of severe shock.
CHEN Yun-yan,WANG Xing-min,SONG Rui et al. Changes of platelet mitochondria in rats with severe hemorrhagic shock and intervention effect of polydatin[J]. CHINESE JOURNAL OF TRAUMA, 2013, 29(9): 882-888.
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