Effects of different doses of Xuebijing injection in treatment of ischemia reperfusion injury in rabbit limbs
State Key Laboratory of Trauma, Burns and Combined injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
Abstract：Objective To investigate effect and mechanism of Xuebijing administration at various concentrations on ischemia reperfusion injury (IRI) of rabbit limbs. Methods Thirty New Zealand rabbits were divided into control group (n=10), Xuebijing group Ⅰ (n=10) and Xuebijing group Ⅱ (n=10) according to random number table. Rabbit models of IRI in lower extremities were established. Each group received corresponding therapy after reperfusion: rabbits in Xuebijing group Ⅰ were firstly administered 4 ml/kg Xuebijing solution and 6 ml/kg isotonic saline; rabbits in Xuebijing group Ⅱ were administered 2 ml/kg Xuebijing solution and 8 ml/kg isotonic saline; rabbits in control group were simply administered 10 ml/kg isotonic saline. Venous blood samples were collected before reperfusion and at 1 h, 2 h, 4 h after reperfusion to measure coagulation parameters (APTT, Fib, INR and PT) and biochemical items (ALB, LDH and CK). Results APTT in Xuebijing group Ⅰ presented obvious improvement at 1 h and 4 h after reperfusion as compared with control group (P<0.01). PT in Xuebijing groups Ⅰ and Ⅱ was significantly longer after reperfusion than that before reperfusion (P<0.05). Fib level in Xuebijing group Ⅰ was much higher at 4 h after reperfusion than that before reperfusion (P<0.05). ALB level at 1 hour after reperfusion showed no statistical differences from that before reperfusion in Xuebijing groups Ⅰ and Ⅱ (P>0.05). LDH and CK levels in Xuebijing group Ⅰwere much lower than those in control group after reperfusion (P<0.05). Conclusions Xuebijing injection relieves limb IRI, with better effect in Xuebijing group Ⅰ than in Xuebijing group Ⅱ. Therapeutic mechanism may be associated with its involvement in adjusting clotting function and mitigating injury of muscle tissues.
ZHANG Liang,DAI Wei,GAO Zhi-ming et al. Effects of different doses of Xuebijing injection in treatment of ischemia reperfusion injury in rabbit limbs[J]. CHINESE JOURNAL OF TRAUMA, 2013, 29(8): 796-799.
［9］Huang YM, Zhao H, Yu X, et al. Damaging mechanism of free radical to the membrane molecules of red blood cell. Acta Biophys Sin, 1997, 13(2):315-323.
