Abstract：Objective To observe bone formation of compound of autologous periosteum cells plus calcium alginate gelatin in animal body and investigate the best mixing ratio of the two components. Methods A total of 96 healthy adult New Zealand rabbits were randomly divided into six groups （Groups A, B, C, D, E and control group）. The excised bilateral periostea from rabbits in each experimental group were all made into 0.4 ml cell suspensions. The compounds were prepared by blending 0.4 ml calcium alginate gelatin with 1, 1/4, 1/16, 1/64 and 1/256 of the periosteum cell suspension respectively, and then were applied to autologous right radial defect area. Gross observation, X-ray examination and histological study were carried out at 2, 4, 8, 12 weeks postoperatively. Levels of serum alkaline phosphatase and serum calcium were determined as well. Results The compounds containing periosteum cell suspension with cell counts of 1/4 or 1/16 and calcium alginate gelatin reached the best osteogenesis. Conclusion Compound of autologous periosteum cell suspension- calcium alginate gelatin induces obvious bone formation and is worthy of clinical application, for it has advantages of satisfactory bone defect repair and easy operation.
［1］Critchlow MA, Bland YS, Ashhurst DE. The effects of age on the response of rabbit periosteal osteogenitor cells to exogenous transforming growth factor-β. J Cell Sci, 1994, 107(2):499-516.
