Arginine vasopressin in combination with norepinephrine for uncontrolled hemorrhagic shock in rats
State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China
Abstract：Objective To investigate the effects of arginine vasopressin (AVP) combined with norepinephrine (NE) in treatment of uncontrolled hemorrhagic shock (UHS) in rats. Methods UHS models were produced in rats and divided into three groups according to the random number table, which were resuscitated with LR equivalent to 1/2 (17.5 ml/kg) of shed blood, LR equivalent to 1/4 (8.75 ml/kg) of shed blood and without LR respectively. Each group was subdivided into six groups: AVP1 (0.04 U/kg) group, AVP2 (0.4 U/kg) group, NE (3 μg/kg) group, AVP1+NE group, AVP2+NE group and LR control group, with 10 rats per group. Effects of single AVP or NE infusion or combined infusion respectively grouped with different doses of LR on survival time and hemodynamics of UHS rats were observed. Results Compared with AVP, NE and AVP+NE groups without LR or with LR equivalent to 1/2 of shed blood respectively, AVP2+NE group with LR equivalent to 1/4 of shed blood provided better main artery pressure (MAP), prolonged survival time and enhanced 4 hour survival rate in treatment of UHS rats. Moreover, survival time and 24 hour survival rate were increased significantly and hemodynamic parameters like MAP, left intraventricular systolic pressure (LVSP) and maximal change rate of left intraventricular pressure (±dp/dt max) were improved after hemostasis. Conclusion AVP (0.04 U/kg)+NE (3 μg/kg) infusion with LR equivalent to 1/4 of shed blood prior to hematosis can win the time for definitive treatment and improve the treatment outcome.
CHEN Xiang-yun,ZHU Yu,TIAN Kun-lun et al. Arginine vasopressin in combination with norepinephrine for uncontrolled hemorrhagic shock in rats[J]. CHINESE JOURNAL OF TRAUMA, 2013, 29(7): 591-596.
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